Overcoming pain is the subject of many researches. The use of herbal medicine for relief of pain was common among people from the ancient time up to date. As some species of Nepeta has been reported to have analgesic effect, therefore, the aim of this study was to determine whether Nepeta glomerulosa, a species native to Iran, has analgesic effect. The effect of different doses of the aqueous and methanolic extracts of the plant (50-2700 mg/kg) on reducing pain was evaluated in mice using formalin test. The aqueous extract at doses of 300-2700 mg/kg and at doses of 100-2700 mg/kg reduced the duration of paw licking, at phase 1 and 2 of the formalin test, respectively, in comparison to vehicle. The methanolic extract reduced the duration of paw licking at dose of 2700 mg/kg and at doses of 900-2700 mg/kg at phase 1 and 2 of the formalin test, respectively, in comparison to vehicle group. The results indicated that the aqueous and methanolic extracts of Nepeta glomerulosa have analgesic effects, but the aqueous extract is more efficient in reducing pain. The future studies should be focused on the aqueous fraction for isolating the active constituent (s) responsible for the analgesic effect.